Biochemical characterization of rice glutelin

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August undefined, 2024

WebSep 1, 2024 · Rice glutelin has high asparagine and glutamine contents (Liu et al., 2011). Thus, it likely has high ability to form fibril aggregates, since Tang et al. (2010) … WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable …

Biochemical and molecular characterization of a rice glutelin allele ...

WebREP-1 was further purified to a single polypeptide of 36 kDa. REP-1 digested in vitro both the acidic and basic subunits of rice glutelin, the major seed storage protein of rice. Determination of the N-terminal amino acid sequence and experiments with protease inhibitors indicated that REP-1 is a cysteine endopeptidase. WebJan 1, 1992 · The 51-kDa protein is the rice glutelin precursor, which is processed to give rise to the 34-37-kDa u-subunit and 21-23-kDa subunit [3,7]. Similarly, the 14-kDa protein has been identified as a rice prolamine [7]. The 25- and 16-kDa proteins were the most abundant polypeptides in the globulin fraction (Fig. 1, lane C). ... Biochemical ... dic-20 high output

Biochemical Characterization of Rice Glutelin 1 Plant …

WebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C. WebThe rice (Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/α-2 encoded by glu4 while forming a new polypeptide of pI6.50/α-1. Experiments were conducted to identify the … WebMay 1, 1985 · The two major subunits of rice glutelin, the acidic (α) and basic (β) polypeptides were purified by chromatofocusing and cation exchange … dic-408w

Biochemical and molecular characterization of a rice glutelin …

WebJun 26, 2007 · The overall electrophoretic profile and alpha glutelin subunits in different rice samples ranged from 12.1 to 100.9 and 32.1 to 34.0 kDa, respectively, while beta glutelin subunits ranged from 23. ... WebApr 1, 2002 · Very little is known about the gene that regulates the biochemical and cellular processes responsible for the formation of PBs. Rice mutants that show variations in the profile patterns of storage proteins have been identified and assigned into four classes (Kumamaru et al., 1988; Ogawa et al., 1989). citi trends backpacksWebJul 1, 2003 · An extensive analysis of the complete rice genome sequence indicates that there are at least 11 glutelin genes (Katsube-Tanaka et al., 2004). However, only GluA … dic 24 form texas

"WebNov 1, 2024 · Characterization of rice DDGS and WS2.2.1. Biochemical characterization. The particle size of DDGS was reduced with mortar and pestle, thereby passing it to 0.5 mm sieve to obtain uniform size of the sample. ... Glutelin and prolamin were the major protein fraction available. A significant percentage of protein was … " - Biochemical characterization of rice glutelin

Biochemical characterization of rice glutelin

Characterization of a new rice glutelin gene - Oxford Academic

WebOct 1, 2024 · The charge density of individual rice glutelin and gum arabic solutions was determined as a function of pH (2.0–7.0) by measuring the zeta potential values in order to confirm the optimum pH for complex coacervation between rice glutelin and gum arabic (Fig. 1 B).The net charge of rice glutelin solutions at pH 3.0 is the most positive (7.2 ± … WebBiochemical Characterization of Rice Glutelin1 Received for publication September 25, 1984 and in revised form January 14, 1985 TUAN-NAN WEN AND DAWN S. LUTHE* …

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WebChickpeas are the third most abundant legume crop worldwide, having a high protein content (14.9–24.6%) with interesting technological properties, thus representing a sustainable alternative to animal proteins. In this study, the surface and structural properties of total (TE) and sequential (ALB, GLO, and GLU) protein fractions isolated from defatted … WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable …

WebUnlike other cereals which accumulate prolamins as their primary nitrogen reserve, the major class of storage proteins in rice and oat are the glutelins and 12S globulins, respectively. These proteins are highly homologous at the primary sequence level to the leguminous US globulins. This similarity in protein structure extends to the molecular ... WebEurope PMC is an archive of life sciences journal literature.

WebThe rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis …

WebRice glutelin cDNA clones isolated from a rice cDNA library constructed from immature endosperm encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. SummaryRice glutelin cDNA clones were isolated from a rice cDNA library …

WebDec 1, 2024 · A spectrofluorometric method was utilized to provide additional information about the interaction of rice glutelin and gallic acid. Fluorescence excitation was carried out at 280 nm, since this wavelength is sensitive to changes in the local environments of tryptophan and tyrosine groups, which provides indirect information about alterations in … dic 24 warningWebNov 2, 2008 · Abstract. A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the … dic 84030blackWebJul 5, 2024 · Background Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the … dic 8603tnw-10WebSep 1, 2012 · The Osborne method comprising of a 2-step in vitro digestion of rice flour was used to extract albumin, globulin, prolamin, and glutelin by using pepsin and trypsin. Subsequently, the antioxidant … Expand dic8360 howdensWebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation … citi trends baby stuffWebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin … dic-407wWebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported … citi trends baytown tx