WebCD40 signal rewires fatty acid and glutamine metabolism for stimulating macrophage anti-tumorigenic functions. View PDF. In Nature Immunology on 1 March 2024 by Liu, P. S., Chen, Y. T., et al.. WebApr 11, 2024 · Then, cells were fixed and permeabilized using Cytofix/Cytoperm with Golgistop Kit (Cat No. 554715; BD biosciences pharmingen, US) according to manufacturer's instruction. After that, cells were incubated with fluorescently conjugated intracellular antibodies.
BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit …
WebBD Cytofix/Cytoperm™ Technical Data Sheet Fixation and Permeabilization Solution Product Information Material Number: 554722 Size: 125 mL Description BD Cytofix/Cytoperm™ solution is supplied as a 1X solution and can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining. … http://pufei.com/static/product/bd/554/192652316a257c47a.pdf small boat cruising the intracoastal
eBioscience™ Intracellular Fixation & Permeabilization …
WebBD Cytofix/Cytoperm™ Fixation and Permeabilization Solution. 1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining. Brand: BD 554722. Additional Details : … WebYou can use BD Cytofix/cytoperm and follow their protocol. Only thing you have to take care is that you should also follow the same procedure for ur isotypic controls, ie, if you are using MPO ... WebNov 15, 2006 · I use the BD Cytofix/Cytoperm™ Plus Kit, which contains a fixation/permeabilization solution containing 4% paraformaldehyde, a permeabilization/washing solution and a protein transport inhibitor (GolgiPlug™ containing brefeldin A). For CFC assays, cells are stimulated for 6 hours with 10 ug/ml peptide or 1 … solution focused therapy essay