WebApplication: Flow cytometry (Routinely Tested) Concentration: 0.5 mg/ml RRID: AB_395627 Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. WebMay 20, 2008 · Here we have optimised a triple colour immunofluorescent staining protocol, utilising an anti-FITC technique, to allow antibodies to macrophage markers to be used simultaneously. We highlight the substantial heterogeneity of cells in both normal liver and spleen that stain for F4/80, CD68, CD11b, and CD11c.
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WebNov 30, 2016 · The antibodies used for labeling of samples were FITC-labeled anti–HLA-DR (L243; Biolegend), anti-CD247 (H146), phycoerythrin (PE)-labeled anti-CD33 (WM53; Biolegend), anti-CD56 (HCD56; Biolegend), APC-labeled anti-CD11b (ICRF44; Biolegend), anti-CD3ϵ (OKT3; Biolegend), and Pacific blue-labeled anti–HLA-DR (L243; Biolegend). WebApr 13, 2024 · For suppression assay, T cells with T-cell activation beads and either CD11b + Ccr1 high or CD11b + Ccr1 low cells were cultured in 1:1 ratio in the heat-inactivated RPMI medium supplemented with ...
WebCD11b-FITC, Bear1, 2 mL, ASR Product No: IM0530U. The CD11b antigen is also known as the integrin αM subunit. It combines with the CD18 antigen (integrin β2 subunit) to … WebCD11b is a type I transmembrane glycoprotein of 170 or 165 kDa under reducing or non-reducing conditions, respectively. CD11b shows 19 potential N-glycosylation sites. Expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen (also known as β2 integrin chain).
WebMar 19, 2024 · In our study, flow cytometric analysis was employed to identify and quantify bone marrow-derived macrophages and their polarized forms using CD11b-FITC, F4/80-APC, CD80-PE or CD206-FITC antibodies. The results demonstrated that M0, M1, and M2 cells respectively amounted to 99.8%, 99.2% and 92% of the total number of cells … WebThe M1/70 antibody reacts with human and mouse CD11b, also known as integrin αalpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin αreceptors …
WebIn Experimental Hematology Oncology on 31 January 2024 by Guo, W., Gong, Q., et al.. Applications: FC/FACS Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. View PDF In Cell Death & Disease on 9 December 2024 by Sun, X., Ni, Y., et al..
WebPerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence. PerCP-Cy5.5 is optimized for … c software consultingWebSee all CD11b primary antibodies Description Rabbit monoclonal [EPR1344] to CD11b Host species Rabbit Tested applications Suitable for: WB, IHC-P more details Species reactivity Reacts with: Mouse, Rat, Human Predicted to work with: Pig, Rhesus monkey Immunogen Synthetic peptide within Human CD11b aa 1-100. The exact sequence is proprietary. c# software developer jobsWebCD11b Antibody, anti-human, REAfinity™ Extended Validation Recombinant Clone: REA713 Type of antibody: Primary antibodies, Recombinant antibodies Isotype: recombinant human IgG1 Applications: FC, MC Alternative names: integrin αM, Mac-1 α, Integrin alpha-M, CR3A Choose your product Select a conjugate APC APC-Vio 770 … ea indWebJan 21, 2024 · This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 301361 301362). more info or order : BioLegend product webpage. eaindray fashionWebApr 10, 2024 · Assessment of cell apoptosis was performed using the cell apoptosis detection kit (Annexin V-FITC/PI, Dojindo) as previously described . Briefly, Panc 02, PANC-1, and HPDE6-C7 cells were seeded into 24-well plates with 500 µL of culture medium at an initial density of 50,000 cells per well. ... DCs (CD45 + CD11b + CD11c +) (G), type 1 ... eainbow plastic filter coverWebAnti-CD11b Antibody Products. CD11b is a reported alias of the human protein 'integrin subunit alpha M', encoded by the gene ITGAM. Anti-CD11b antibodies are available from several suppliers. The full protein is reported to be 1152 amino acid residues in length. Based on antigen name, there may be canine, porcine, monkey, mouse and rat variants ... c++ software developer jobsWebFeb 20, 2015 · VA Directive 6518 4 f. The VA shall identify and designate as “common” all information that is used across multiple Administrations and staff offices to serve VA Customers or manage the ea indir