WebJun 23, 2024 · By two rounds of co-encapsulation and fluorescence activated sorting using a standard FACS instrument, mIL-3 secreting yeast cells could be rapidly and efficiently enriched and selected from... WebMar 7, 2024 · The droplets containing the CFPS reaction mix were sorted by fluorescence-activated droplet sorting (FADS) using an On-chip Sort instrument (On-chip Biotechnologies) in Chip-Z1001 chips. The sheath fluid used was 0.1% Pico-Surf 1 in HFE-7500, and low-density mineral oil (Sigma) was added to the collection reservoir to trap …
Ankit Jain – Postdoctoral Researcher – ETH Zürich
WebJun 3, 2024 · Droplet-based microfluidics is a groundbreaking technology for the miniaturization and automation of biochemical assays at a ultra-high throughput. 2 The technology is key in a wide range of applications, for example for protein engineering, 3–5 cell and microorganism screening, 6–8 sequencing 9,10 or molecular diagnostics. 11 WebApr 7, 2024 · The sorting algorithm was programmed by LabVIEW. When a droplet passed the thresholds, a high voltage was triggered to pull the droplet into the sorting channel. The sorted droplet moved downstream toward the second point of detection for double confirmation of the fluorescent signals. When the sorted droplet passed the thresholds … imdb terence morgan
Fluorescence-Activated Droplet Sorting of Polyethylene ... - bioRxiv
WebDec 19, 2024 · In this study, we developed an ultra-high throughput screening pipeline for lipase-producing strains based on fluorescence-activated droplet sorting (FADS) using a compact optical system that could be easily set up in an alignment-free manner. WebHowever, the throughput of fluorescence lifetime activated droplet sorting is orders of magnitude lower than that of fluorescence … WebFeb 15, 2024 · Among them, microfluidic fluorescence-activated droplet sorting (FADS) was introduced to dramatically simplify the operations, improve sorting efficiency and flexibility, and reduce the cost of large library screening (Baret et … list of miss rheingold winners